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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Roundup Ready™ alfalfa
EN
J101 x J163
Yes
MON-ØØ1Ø1-8 × MON-ØØ163-7
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Organization:Monsanto ()800 North Lindbergh Blvd.St. Louis, MO
63167, United States of AmericaPhone: + 1 314 694-1000,Fax: +1 314 694-3080,Email:Website: http://www.monsanto.com,
Medicago sativa, alfalfa (also known as lucerne) is a perennial herbaceous legume. Lines J101 and J163 contain the epsps gene from Agrobacterium tumefaciens strain CP4 to allow for the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12100-4 Organism Medicago sativa (Alfalfa, Lucerne, MEDSV)Crops
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BCH-LMO-SCBD-15330-6 Living Modified Organism MON-ØØ1Ø1-8 - Roundup Ready™ alfalfaMonsanto | Resistance to herbicides (Glyphosate)
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BCH-LMO-SCBD-15331-7 Living Modified Organism MON-ØØ163-7 - Roundup Ready™ alfalfaMonsanto | Resistance to herbicides (Glyphosate)
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PV-MSHT4
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- Cross breeding
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Protein coding sequence | Resistance to herbicides (Glyphosate)
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BCH-GENE-SCBD-101507-5 FMV 34S promoter | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)Promoter
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BCH-GENE-SCBD-103901-2 HSP 70 5' untranslated leader sequence | Petunia hybrida (Petunia, PETHY)Leader
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BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Transit signal
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BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)Terminator
A stacked line of alfalfa with tolerance to glyphosate through introduction of epsps genes from Agrobacterium tumefaciens strain CP4.
Information on the inserted DNA sequences
The T-DNA portion of this plasmid vector contained one cp4 epsps gene expression cassette coding for glyphosate tolerance. The cp4 epsps gene was linked to a chloroplast transit peptide (cpt2) sequence, derived from the Arabidopsis thaliana epsps gene. The expression of the cp4 epsps gene was regulated by an enhanced 35S promoter from the Figwort Mosaic Virus, and the petunia heat shock protein intron (HSP) 70 5’ untranslated leader sequence. The transcriptional termination sequences were derived from the pea (Pisum sativum) ribulose-1,5-biphosphate carboxylase (Rubisco) small subunit E9 gene.
Vector information
Plant transformation was achieved with the plasmid vector PV-MSHT4, a binary vector which contains a region of DNA (T-DNA), which has one cp4 epsps expression cassette under the control of a constitutive promoter flanked by left and right border sequences which is approximately 3.8 Kb. No additional elements from the transformation vector PV-MSHT4, linked or unlinked to the intact gene cassette were detected in the genome of events J101 and J163.
EN
Information on the inserted DNA sequences
The T-DNA portion of this plasmid vector contained one cp4 epsps gene expression cassette coding for glyphosate tolerance. The cp4 epsps gene was linked to a chloroplast transit peptide (cpt2) sequence, derived from the Arabidopsis thaliana epsps gene. The expression of the cp4 epsps gene was regulated by an enhanced 35S promoter from the Figwort Mosaic Virus, and the petunia heat shock protein intron (HSP) 70 5’ untranslated leader sequence. The transcriptional termination sequences were derived from the pea (Pisum sativum) ribulose-1,5-biphosphate carboxylase (Rubisco) small subunit E9 gene.
Vector information
Plant transformation was achieved with the plasmid vector PV-MSHT4, a binary vector which contains a region of DNA (T-DNA), which has one cp4 epsps expression cassette under the control of a constitutive promoter flanked by left and right border sequences which is approximately 3.8 Kb. No additional elements from the transformation vector PV-MSHT4, linked or unlinked to the intact gene cassette were detected in the genome of events J101 and J163.
EN
- Food
- Feed
- MON-ØØ1Ø1-8 - CropLife International Detection Methods Database ( CropLife ) [ English ]
- MON-ØØ163-7 - CropLife International Detection Methods Database ( CropLife ) [ English ]
EN
The genetically engineered plant contains a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows it to survive the otherwise lethal application of glyphosate. The EPSPS gene that was introduced into J101 and J163 was isolated from the common soil bacterium Agrobacterium tumefaciens, strain CP4 and the form of EPSPS enzyme produced by this gene is tolerant to glyphosate.
This plant is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment.
EN
This plant is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment.
- USDA/APHIS Environmental Assessment 04-110-01p [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 4 | |
| Country's Decision or any other Communication | LMO identification | 5 | |
| Risk Assessment generated by an independent or non-regulatory process | Living modified organism(s) | 1 | |
